@article {pearl_mizrahi_community_2023,
	title = {Community interactions drive the evolution of antibiotic tolerance in bacteria},
	journal = {Proceedings of the National Academy of Sciences},
	volume = {120},
	number = {3},
	year = {2023},
	pages = {e2209043119},
	abstract = {The emergence of antibiotic tolerance (prolonged survival against exposure) in natural bacterial populations is a major concern. Since it has been studied primarily in isogenic populations, we do not yet understand how ecological interactions in a diverse community impact the evolution of tolerance. To address this, we studied the evolutionary dynamics of a synthetic bacterial community composed of two interacting strains. In this community, an antibiotic-resistant strain protected the other, susceptible strain by degrading the antibiotic ampicillin in the medium. Surprisingly, we found that in the presence of antibiotics, the susceptible strain evolved tolerance. Tolerance was typified by an increase in survival as well as an accompanying decrease in the growth rate, highlighting a trade-off between the two. A simple mathematical model explained that the observed decrease in the death rate, even when coupled with a decreased growth rate, is beneficial in a community with weak protective interactions. In the presence of strong interactions, the model predicted that the trade-off would instead be detrimental, and tolerance would not emerge, which we experimentally verified. By whole genome sequencing the evolved tolerant isolates, we identified two genetic hot spots which accumulated mutations in parallel lines, suggesting their association with tolerance. Our work highlights that ecological interactions can promote antibiotic tolerance in bacterial communities, which has remained understudied.},
	issn = {0027-8424, 1091-6490},
	doi = {10.1073/pnas.2209043119},
	url = {https://pnas.org/doi/10.1073/pnas.2209043119},
	author = {Pearl Mizrahi, Sivan and Goyal, Akshit and Gore, Jeff}
}
